鱿鱼(Squid)肝脏蛋白酶解物中ACE活性抑制肽的分离纯化及活性分析     

梁佳  谢超  林琳  黄菊  王婷  俞群娣 《海洋与湖沼》2015,46(5):1175-1179

为提高鱿鱼加工下脚料的利用率,以秘鲁鱿鱼肝脏为研究对象,采用生物酶解技术和凝胶蛋白分离技术等对其进行研究。研究结果表明:鱿鱼肝脏蛋白酶解液经过超滤处理后(分子质量为20k Da),用Sephadex G-100初步分离,得到五个组分,对分离的组分进行研究,结果表明降血压活性能力最强的是组分二,测定其半抑制浓度(IC50)为1.8mg/m L。对组分二进行活性条件分析,结构表明其ACE抑制活性在温度0—100°C、p H 1—12之间基本没有变化。最后用DEAE阴离子交换柱和Sephadex LH-20对组分二继续分离纯化,得到组分六和组分七,测定其半抑制浓度(IC50)分别达到1.52 mg/m L和2.16 mg/m L。本研究可为利用鱿鱼加工下脚料开发降血压活性肽产品提供理论基础。  相似文献   

冲击荷载作用下新型延展式张力腿平台时域分析     

巩超  黄维平 《海洋工程》2015,33(1):24-30

延展式张力腿平台是一种适用于深水油气开采的海洋平台结构。提出一种新型平台由四个立柱和一个环形浮箱连接而成,环形浮箱是由四个箱型梁焊接而成。根据给出的南海海域的海况条件和相应的规范计算结构的环境载荷,运用SESAM软件Deep C模块,考虑风、浪、流等环境荷载的作用和张力腿系统的非线性影响,进行了时域耦合分析,得到了平台的运动位移时程、张力腿系统张力时程和张力极值等。模拟波浪破碎作为冲击载荷,得到张力腿平台在冲击荷载作用下的运动位移时程、张力腿系统的张力时程。研究结果表明:该新型平台具有良好的运动响应,张力筋腱应力满足安全要求;在冲击载荷作用下新型平台的运动响应也满足要求。  相似文献   

Ultimate Load Capacity of Offshore Pipeline with Arbitrary Shape Corrosion Defects     

陈严飞  张娟  张宏  刘啸奔  李昕  周晶  曹静 《海洋工程》2015,29(2):241-252

A set of generalized solutions are proposed for estimating ultimate load capacity of pipeline with arbitrary corrosion shapes subjected to combined internal pressure, axial force and bending moment. Isotropic and anisotropic material characteristics in longitudinal and circumferential direction of pipeline are also considered in the proposed equations. Simplified numerical method is used to solve the generalized expressions. The comparisons of numerical results based generalized solutions and full-scale experimental results are carried out. The predicted results agree reasonably well with the experiment results. Meanwhile, the effects of corrosion shapes and locations on the ultimate load capacity are studied.  相似文献   

An Experimental Study on A Trapezoidal Pendulum Wave Energy Converter in Regular Waves     

王冬姣  邱守强  叶家玮 《海洋工程》2015,29(4):623-632

Experimental studies were conducted on a trapezoidal pendulum wave energy converter in regular waves. To obtain the incident wave height, the analytical method (AM) was used to separate the incident and reflected waves propagating in a wave flume by analysing wave records measured at two locations. The response amplitude operator (RAO), primary conversion efficiency and the total conversion efficiency of the wave energy converter were studied; furthermore, the power take-off damping coefficients corresponding to the load resistances in the experiment were also obtained. The findings demonstrate that the natural period for a pendulum wave energy converter is relatively large. A lower load resistance gives rise to a larger damping coefficient. The model shows relatively higher wave energy conversion efficiency in the range of 1.0?1.2 s for the incident wave period. The maximum primary conversion efficiency achieved was 55.5%, and the maximum overall conversion efficiency was 39.4%.  相似文献   

AutoCAD自动加载VBA程序研究     

魏磊  田磊 《测绘与空间地理信息》2015,(9)

VBA语言是进行AutoCAD二次开发的重要手段之一。本文主要探讨了AutoCAD平台下如何自动加载VBA程序,详细介绍了当前常用的自动加载VBA程序的方法,对这些方法在使用过程中的优点和局限性进行了对比分析,最后引入了使用代码操作注册表加载VBA程序的方法。  相似文献   

硫代硫酸钠沉淀法富集铜冶炼废酸中铼的实验研究   总被引：2，自引：0，他引：2  

王永斌  黄建芬  栗威  梁富明 《岩石矿物学杂志》2015,34(1):110-116

白银有色集团铜业公司冶炼废酸量每年约为1×105m3,其中铼含量为5~70 mg/L,为综合利用废酸中的铼,采用硫代硫酸钠作为特效沉淀剂,对该沉淀剂加入量、反应时间、反应温度以及废酸中铼含量对铼沉淀率的影响做了较系统的实验研究,得到较优化的沉淀条件。进一步的中试放大实验取得了良好的技术指标:富铼渣泥中含铼达1.66%,铼的回收率达到99.8%。在此基础上,对沉淀剂的氧化还原反应机理进行了探讨。本方法工艺简单,铼沉淀率高,环境友好,较适用于废酸中铼的高效提取。  相似文献   

黄河内蒙古河段输沙量与淤积量计算方法   总被引：2，自引：0，他引：2       下载免费PDF全文

吴保生  刘可晶  申红彬  周丽艳 《水科学进展》2015,26(3):311-321

基于多沙河流"多来多排"输沙基本公式,建立了考虑上站来沙量、前期累计淤积量、临界输沙水量及干支流泥沙粒径影响的输沙量一般表达式。进而根据黄河内蒙古河段1953—2010年实测水沙资料,得到了内蒙古巴彦高勒—头道拐全河段及巴彦高勒—三湖河口和三湖河口—头道拐分河段的年输沙量公式,并根据输沙率法采用建立的输沙量公式计算了各河段1953—2010年的逐年冲淤量。选取三湖河口—头道拐河段的计算结果分析表明,在输沙基本公式基础上增加前期累计淤积量、临界输沙水量及干支流泥沙粒径参数,所得公式计算的年冲淤量与实测值之间的相关系数R2由基本公式的0.41依次提高到0.50、0.75和0.80,说明在基本公式基础上进一步考虑这些参数的必要性。此外,全河及分河段输沙公式计算所得输沙量及各河段冲淤量和累计淤积量与实测值的符合程度均较好,表明建立的输沙公式能够用于不同水沙条件下的输沙量和冲淤量计算,可为分析内蒙古河段的输沙特性和长期淤积趋势提供参考。  相似文献   

竖向高荷载作用下大直径超长钻孔灌注桩承载性能试验     

桑伟锋 《世界地质》2020,39(1):127-134

通过机场—西华高速大直径超长钻孔灌注桩大吨位竖向单桩静载试验,分析了该地区大直径超长钻孔灌注桩的承载性状以及荷载传递机理。试验结果表明:试桩的Q-S曲线呈缓变型,桩端承载力占总荷载的比例均<10%,即均表现为摩擦桩特性;试桩的侧摩阻力自上而下逐步发挥,侧摩阻力和桩端阻力异步发挥且互相耦合;大直径超长钻孔灌注桩桩侧摩阻力的发挥与土层性质、土层埋深及桩顶荷载水平有关;在高荷载作用下桩侧上部土层摩阻力具有不同程度的软化现象,而中下部土层侧摩阻力具有不同程度的强化现象,甚至即使在最大加载情况下,桩身下部土层的侧摩阻力也并未完全发挥,因此在根据规范计算超长桩承载力时,不同深度土层的侧摩阻力应乘以相应不同的修正系数。  相似文献   

Effects of long-term cyclic horizontal loading on bucket foundations in saturated loose sand     

《Applied Ocean Research》2019

A 1-g model experimental study was conducted to investigate the accumulated rotations and unloading stiffness of bucket foundations in saturated loose sand. One-way horizontal cyclic loading was applied to model bucket foundations with embedment ratios 0.5 and 1.0. Up to 10⁴ cycles of loading were applied at a frequency of 0.2 Hz varying load amplitudes. The accumulated rotation of the bucket foundations increased with the number of cycles and the load amplitudes. Empirical equations were proposed to describe the accumulated rotation of the foundations. The unloading stiffness of foundations increased with the number of cycles but decreased with an increase in load amplitude. The initial unloading stiffness of L/D = 1.0 (L is skirt length; D is foundation diameter) was approximately twice that of L/D = 0.5. Excess pore water pressure difference of 50% was observed between L/D = 0.5 and 1.0. The suction and static capacity of the bucket increased with increase of bucket embedment ratio with a difference of 69.5% and 73.6% respectively between L/D = 0.5 and 1.0.  相似文献   

Adaptive analysis of infinite beams dynamics problems using the periodic configuration update method in the time domain          下载免费PDF全文

Reda Mezeh  Marwan Sadek  Fadi Hage Chehade  Isam Shahrour 《国际地质力学数值与分析法杂志》2018,42(4):618-635

This paper proposes a new approach for the assessment of the dynamic response of continuously supported infinite beams under high‐speed moving loads. A change in the representation of equations of motion in the dynamics of discrete structures is proposed to obtain an improved accuracy of the numerical integration in the time domain. The proposed numerical method called the “periodic configuration update” or “PCU method” is applied to solve the problem of a vertical moving harmonic load on an infinite classical Euler‐Bernoulli beam resting on a continuous viscoelastic foundation. This study shows the superiority of the proposed method in comparison with other methods presented in the literature that suffer from the material time derivative, i.e., convective terms, that arises from the Galilean transformation. To confront this numerical problem, the PCU method retains the principle of the spatial follow of loads while zeroing the relative velocity with the traversed beam via a step‐by‐step adaptive integration of the equation of structural dynamics. The dynamic load is modeled with high theoretical velocities that can reach the critical velocity of the studied beam with different angular frequencies belonging to moderate frequency range. A parametric study is carried out to analyze the influence of key parameters on the convergence. The obtained results show a high efficiency of the PCU method for solving these types of problems relative to the dynamics of high speed trains/tracks.  相似文献